Proximity Ligation Assay:Article Title: Solid-phase proximity ligation assays for individual or parallel protein analyses with readout via real-time PCR or sequencing.
Article Snippet: Streptavidin-conjugated oligonucleotides were ordered from Solulink PLA probes (see INTRODUCTION) Potassium acetate Potassium chloride (Merck, cat. no. 1.04936.1000) Potassium dihydrogen phosphate (Merck, cat. no. 1.04873.1000) Salmon sperm DNA (Life Technologies, cat. no. 15632-011) Sodium chloride (Merck, cat. no. 1.06404.1000) Disodium hydrogen phosphate dihydrate (Merck, cat. no. 1.06580.1000) Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC; Thermo Scientific, cat. no. 22322) SYBR Green I nucleic acid gel stain, 10,000× concentrate in DMSO (Life Technologies, cat. no. S-7563) T4 DNA ligase, 1 U μl − 1 (Thermo Scientific, cat. no. EL0016) Tween-20 (Sigma-Aldrich, cat. no. P9416) Uracil-DNA glycosylase (UDG), 1 U μl − 1 (Thermo Scientific, cat. no. EN0362) UltraPure dithiothreitol (Life Technologies, cat. no. 15508-013) Zeba Spin desalting columns, 7 K MWCO, 0.5 ml (Thermo Scientific, cat. no. 89882) Zeba Spin desalting plates, 7 K MWCO (Thermo Scientific, cat. no. 89808) Trizma base EQUIPMENT Beaker, 5 liters PCR strip, eight wells (e.g., Applied Biosystems, cat. no. 4316567) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • PCR plate, 96 wells (e.g., Sarstedt, cat. no. 72.1978.202; Thermo Scientific, cat. no. 732-4828) 2720 thermal cycler (Applied Biosystems, cat. no. 4359659) 7900 HT fast real-time PCR system (Applied Biosystems, cat. no. 4329001) Cap strips (8 caps per strip; e.g., Applied Biosystems, cat. no. 4323032) DynaMag-2 magnet (Life Technologies, cat. no. 12321D), or similar magnetic stand for microcentrifuge tubes DynaMag-96 side, 96-well-plate magnet (Life Technologies, cat. no. 12331D) E-gel agarose gel electrophoresis system (Life Technologies, cat. no. G6500) E-gel precasted agarose gel (Life Technologies, cat. no. G402002) Hydra 96 system (Robbins Scientific) Icebox Magnetic stir bar MicroAmp optical 384-well reaction plate with bar code (Applied Biosystems, cat. no. 4309849) Multichannel pipette (Eppendorf International) M×3000P qPCR system (Agilent Technologies) Plate centrifuge (Eppendorf International) Rotator (Grant Bio PTR-30 360-degree vertical multifunction rotator, or similar) Self-adhesive PCR film (Sarstedt, cat. no. 95.1994) Slide-A-Lyzer MINI dialysis devices, 7 K MWCO (Thermo Scientific, cat. no. 69562) Vortex mixer REAGENT SETUP PBS buffer, 1×, pH 7.4 PBS buffer contains 137 mM NaCl, 2.7 mM KCl and 10 mM phosphate.
SYBR Green Assay:Article Title: Solid-phase proximity ligation assays for individual or parallel protein analyses with readout via real-time PCR or sequencing.
Article Snippet: Streptavidin-conjugated oligonucleotides were ordered from Solulink PLA probes (see INTRODUCTION) Potassium acetate Potassium chloride (Merck, cat. no. 1.04936.1000) Potassium dihydrogen phosphate (Merck, cat. no. 1.04873.1000) Salmon sperm DNA (Life Technologies, cat. no. 15632-011) Sodium chloride (Merck, cat. no. 1.06404.1000) Disodium hydrogen phosphate dihydrate (Merck, cat. no. 1.06580.1000) Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC; Thermo Scientific, cat. no. 22322) SYBR Green I nucleic acid gel stain, 10,000× concentrate in DMSO (Life Technologies, cat. no. S-7563) T4 DNA ligase, 1 U μl − 1 (Thermo Scientific, cat. no. EL0016) Tween-20 (Sigma-Aldrich, cat. no. P9416) Uracil-DNA glycosylase (UDG), 1 U μl − 1 (Thermo Scientific, cat. no. EN0362) UltraPure dithiothreitol (Life Technologies, cat. no. 15508-013) Zeba Spin desalting columns, 7 K MWCO, 0.5 ml (Thermo Scientific, cat. no. 89882) Zeba Spin desalting plates, 7 K MWCO (Thermo Scientific, cat. no. 89808) Trizma base EQUIPMENT Beaker, 5 liters PCR strip, eight wells (e.g., Applied Biosystems, cat. no. 4316567) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • PCR plate, 96 wells (e.g., Sarstedt, cat. no. 72.1978.202; Thermo Scientific, cat. no. 732-4828) 2720 thermal cycler (Applied Biosystems, cat. no. 4359659) 7900 HT fast real-time PCR system (Applied Biosystems, cat. no. 4329001) Cap strips (8 caps per strip; e.g., Applied Biosystems, cat. no. 4323032) DynaMag-2 magnet (Life Technologies, cat. no. 12321D), or similar magnetic stand for microcentrifuge tubes DynaMag-96 side, 96-well-plate magnet (Life Technologies, cat. no. 12331D) E-gel agarose gel electrophoresis system (Life Technologies, cat. no. G6500) E-gel precasted agarose gel (Life Technologies, cat. no. G402002) Hydra 96 system (Robbins Scientific) Icebox Magnetic stir bar MicroAmp optical 384-well reaction plate with bar code (Applied Biosystems, cat. no. 4309849) Multichannel pipette (Eppendorf International) M×3000P qPCR system (Agilent Technologies) Plate centrifuge (Eppendorf International) Rotator (Grant Bio PTR-30 360-degree vertical multifunction rotator, or similar) Self-adhesive PCR film (Sarstedt, cat. no. 95.1994) Slide-A-Lyzer MINI dialysis devices, 7 K MWCO (Thermo Scientific, cat. no. 69562) Vortex mixer REAGENT SETUP PBS buffer, 1×, pH 7.4 PBS buffer contains 137 mM NaCl, 2.7 mM KCl and 10 mM phosphate.
Staining:Article Title: Solid-phase proximity ligation assays for individual or parallel protein analyses with readout via real-time PCR or sequencing.
Article Snippet: Streptavidin-conjugated oligonucleotides were ordered from Solulink PLA probes (see INTRODUCTION) Potassium acetate Potassium chloride (Merck, cat. no. 1.04936.1000) Potassium dihydrogen phosphate (Merck, cat. no. 1.04873.1000) Salmon sperm DNA (Life Technologies, cat. no. 15632-011) Sodium chloride (Merck, cat. no. 1.06404.1000) Disodium hydrogen phosphate dihydrate (Merck, cat. no. 1.06580.1000) Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC; Thermo Scientific, cat. no. 22322) SYBR Green I nucleic acid gel stain, 10,000× concentrate in DMSO (Life Technologies, cat. no. S-7563) T4 DNA ligase, 1 U μl − 1 (Thermo Scientific, cat. no. EL0016) Tween-20 (Sigma-Aldrich, cat. no. P9416) Uracil-DNA glycosylase (UDG), 1 U μl − 1 (Thermo Scientific, cat. no. EN0362) UltraPure dithiothreitol (Life Technologies, cat. no. 15508-013) Zeba Spin desalting columns, 7 K MWCO, 0.5 ml (Thermo Scientific, cat. no. 89882) Zeba Spin desalting plates, 7 K MWCO (Thermo Scientific, cat. no. 89808) Trizma base EQUIPMENT Beaker, 5 liters PCR strip, eight wells (e.g., Applied Biosystems, cat. no. 4316567) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • PCR plate, 96 wells (e.g., Sarstedt, cat. no. 72.1978.202; Thermo Scientific, cat. no. 732-4828) 2720 thermal cycler (Applied Biosystems, cat. no. 4359659) 7900 HT fast real-time PCR system (Applied Biosystems, cat. no. 4329001) Cap strips (8 caps per strip; e.g., Applied Biosystems, cat. no. 4323032) DynaMag-2 magnet (Life Technologies, cat. no. 12321D), or similar magnetic stand for microcentrifuge tubes DynaMag-96 side, 96-well-plate magnet (Life Technologies, cat. no. 12331D) E-gel agarose gel electrophoresis system (Life Technologies, cat. no. G6500) E-gel precasted agarose gel (Life Technologies, cat. no. G402002) Hydra 96 system (Robbins Scientific) Icebox Magnetic stir bar MicroAmp optical 384-well reaction plate with bar code (Applied Biosystems, cat. no. 4309849) Multichannel pipette (Eppendorf International) M×3000P qPCR system (Agilent Technologies) Plate centrifuge (Eppendorf International) Rotator (Grant Bio PTR-30 360-degree vertical multifunction rotator, or similar) Self-adhesive PCR film (Sarstedt, cat. no. 95.1994) Slide-A-Lyzer MINI dialysis devices, 7 K MWCO (Thermo Scientific, cat. no. 69562) Vortex mixer REAGENT SETUP PBS buffer, 1×, pH 7.4 PBS buffer contains 137 mM NaCl, 2.7 mM KCl and 10 mM phosphate.
Polymerase Chain Reaction:Article Title: Solid-phase proximity ligation assays for individual or parallel protein analyses with readout via real-time PCR or sequencing.
Article Snippet: Streptavidin-conjugated oligonucleotides were ordered from Solulink PLA probes (see INTRODUCTION) Potassium acetate Potassium chloride (Merck, cat. no. 1.04936.1000) Potassium dihydrogen phosphate (Merck, cat. no. 1.04873.1000) Salmon sperm DNA (Life Technologies, cat. no. 15632-011) Sodium chloride (Merck, cat. no. 1.06404.1000) Disodium hydrogen phosphate dihydrate (Merck, cat. no. 1.06580.1000) Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC; Thermo Scientific, cat. no. 22322) SYBR Green I nucleic acid gel stain, 10,000× concentrate in DMSO (Life Technologies, cat. no. S-7563) T4 DNA ligase, 1 U μl − 1 (Thermo Scientific, cat. no. EL0016) Tween-20 (Sigma-Aldrich, cat. no. P9416) Uracil-DNA glycosylase (UDG), 1 U μl − 1 (Thermo Scientific, cat. no. EN0362) UltraPure dithiothreitol (Life Technologies, cat. no. 15508-013) Zeba Spin desalting columns, 7 K MWCO, 0.5 ml (Thermo Scientific, cat. no. 89882) Zeba Spin desalting plates, 7 K MWCO (Thermo Scientific, cat. no. 89808) Trizma base EQUIPMENT Beaker, 5 liters PCR strip, eight wells (e.g., Applied Biosystems, cat. no. 4316567) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • PCR plate, 96 wells (e.g., Sarstedt, cat. no. 72.1978.202; Thermo Scientific, cat. no. 732-4828) 2720 thermal cycler (Applied Biosystems, cat. no. 4359659) 7900 HT fast real-time PCR system (Applied Biosystems, cat. no. 4329001) Cap strips (8 caps per strip; e.g., Applied Biosystems, cat. no. 4323032) DynaMag-2 magnet (Life Technologies, cat. no. 12321D), or similar magnetic stand for microcentrifuge tubes DynaMag-96 side, 96-well-plate magnet (Life Technologies, cat. no. 12331D) E-gel agarose gel electrophoresis system (Life Technologies, cat. no. G6500) E-gel precasted agarose gel (Life Technologies, cat. no. G402002) Hydra 96 system (Robbins Scientific) Icebox Magnetic stir bar MicroAmp optical 384-well reaction plate with bar code (Applied Biosystems, cat. no. 4309849) Multichannel pipette (Eppendorf International) M×3000P qPCR system (Agilent Technologies) Plate centrifuge (Eppendorf International) Rotator (Grant Bio PTR-30 360-degree vertical multifunction rotator, or similar) Self-adhesive PCR film (Sarstedt, cat. no. 95.1994) Slide-A-Lyzer MINI dialysis devices, 7 K MWCO (Thermo Scientific, cat. no. 69562) Vortex mixer REAGENT SETUP PBS buffer, 1×, pH 7.4 PBS buffer contains 137 mM NaCl, 2.7 mM KCl and 10 mM phosphate.
Stripping Membranes:Article Title: Solid-phase proximity ligation assays for individual or parallel protein analyses with readout via real-time PCR or sequencing.
Article Snippet: Streptavidin-conjugated oligonucleotides were ordered from Solulink PLA probes (see INTRODUCTION) Potassium acetate Potassium chloride (Merck, cat. no. 1.04936.1000) Potassium dihydrogen phosphate (Merck, cat. no. 1.04873.1000) Salmon sperm DNA (Life Technologies, cat. no. 15632-011) Sodium chloride (Merck, cat. no. 1.06404.1000) Disodium hydrogen phosphate dihydrate (Merck, cat. no. 1.06580.1000) Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC; Thermo Scientific, cat. no. 22322) SYBR Green I nucleic acid gel stain, 10,000× concentrate in DMSO (Life Technologies, cat. no. S-7563) T4 DNA ligase, 1 U μl − 1 (Thermo Scientific, cat. no. EL0016) Tween-20 (Sigma-Aldrich, cat. no. P9416) Uracil-DNA glycosylase (UDG), 1 U μl − 1 (Thermo Scientific, cat. no. EN0362) UltraPure dithiothreitol (Life Technologies, cat. no. 15508-013) Zeba Spin desalting columns, 7 K MWCO, 0.5 ml (Thermo Scientific, cat. no. 89882) Zeba Spin desalting plates, 7 K MWCO (Thermo Scientific, cat. no. 89808) Trizma base EQUIPMENT Beaker, 5 liters PCR strip, eight wells (e.g., Applied Biosystems, cat. no. 4316567) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • PCR plate, 96 wells (e.g., Sarstedt, cat. no. 72.1978.202; Thermo Scientific, cat. no. 732-4828) 2720 thermal cycler (Applied Biosystems, cat. no. 4359659) 7900 HT fast real-time PCR system (Applied Biosystems, cat. no. 4329001) Cap strips (8 caps per strip; e.g., Applied Biosystems, cat. no. 4323032) DynaMag-2 magnet (Life Technologies, cat. no. 12321D), or similar magnetic stand for microcentrifuge tubes DynaMag-96 side, 96-well-plate magnet (Life Technologies, cat. no. 12331D) E-gel agarose gel electrophoresis system (Life Technologies, cat. no. G6500) E-gel precasted agarose gel (Life Technologies, cat. no. G402002) Hydra 96 system (Robbins Scientific) Icebox Magnetic stir bar MicroAmp optical 384-well reaction plate with bar code (Applied Biosystems, cat. no. 4309849) Multichannel pipette (Eppendorf International) M×3000P qPCR system (Agilent Technologies) Plate centrifuge (Eppendorf International) Rotator (Grant Bio PTR-30 360-degree vertical multifunction rotator, or similar) Self-adhesive PCR film (Sarstedt, cat. no. 95.1994) Slide-A-Lyzer MINI dialysis devices, 7 K MWCO (Thermo Scientific, cat. no. 69562) Vortex mixer REAGENT SETUP PBS buffer, 1×, pH 7.4 PBS buffer contains 137 mM NaCl, 2.7 mM KCl and 10 mM phosphate.
Real-time Polymerase Chain Reaction:Article Title: Solid-phase proximity ligation assays for individual or parallel protein analyses with readout via real-time PCR or sequencing.
Article Snippet: Streptavidin-conjugated oligonucleotides were ordered from Solulink PLA probes (see INTRODUCTION) Potassium acetate Potassium chloride (Merck, cat. no. 1.04936.1000) Potassium dihydrogen phosphate (Merck, cat. no. 1.04873.1000) Salmon sperm DNA (Life Technologies, cat. no. 15632-011) Sodium chloride (Merck, cat. no. 1.06404.1000) Disodium hydrogen phosphate dihydrate (Merck, cat. no. 1.06580.1000) Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC; Thermo Scientific, cat. no. 22322) SYBR Green I nucleic acid gel stain, 10,000× concentrate in DMSO (Life Technologies, cat. no. S-7563) T4 DNA ligase, 1 U μl − 1 (Thermo Scientific, cat. no. EL0016) Tween-20 (Sigma-Aldrich, cat. no. P9416) Uracil-DNA glycosylase (UDG), 1 U μl − 1 (Thermo Scientific, cat. no. EN0362) UltraPure dithiothreitol (Life Technologies, cat. no. 15508-013) Zeba Spin desalting columns, 7 K MWCO, 0.5 ml (Thermo Scientific, cat. no. 89882) Zeba Spin desalting plates, 7 K MWCO (Thermo Scientific, cat. no. 89808) Trizma base EQUIPMENT Beaker, 5 liters PCR strip, eight wells (e.g., Applied Biosystems, cat. no. 4316567) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • PCR plate, 96 wells (e.g., Sarstedt, cat. no. 72.1978.202; Thermo Scientific, cat. no. 732-4828) 2720 thermal cycler (Applied Biosystems, cat. no. 4359659) 7900 HT fast real-time PCR system (Applied Biosystems, cat. no. 4329001) Cap strips (8 caps per strip; e.g., Applied Biosystems, cat. no. 4323032) DynaMag-2 magnet (Life Technologies, cat. no. 12321D), or similar magnetic stand for microcentrifuge tubes DynaMag-96 side, 96-well-plate magnet (Life Technologies, cat. no. 12331D) E-gel agarose gel electrophoresis system (Life Technologies, cat. no. G6500) E-gel precasted agarose gel (Life Technologies, cat. no. G402002) Hydra 96 system (Robbins Scientific) Icebox Magnetic stir bar MicroAmp optical 384-well reaction plate with bar code (Applied Biosystems, cat. no. 4309849) Multichannel pipette (Eppendorf International) M×3000P qPCR system (Agilent Technologies) Plate centrifuge (Eppendorf International) Rotator (Grant Bio PTR-30 360-degree vertical multifunction rotator, or similar) Self-adhesive PCR film (Sarstedt, cat. no. 95.1994) Slide-A-Lyzer MINI dialysis devices, 7 K MWCO (Thermo Scientific, cat. no. 69562) Vortex mixer REAGENT SETUP PBS buffer, 1×, pH 7.4 PBS buffer contains 137 mM NaCl, 2.7 mM KCl and 10 mM phosphate.
Agarose Gel Electrophoresis:Article Title: Solid-phase proximity ligation assays for individual or parallel protein analyses with readout via real-time PCR or sequencing.
Article Snippet: Streptavidin-conjugated oligonucleotides were ordered from Solulink PLA probes (see INTRODUCTION) Potassium acetate Potassium chloride (Merck, cat. no. 1.04936.1000) Potassium dihydrogen phosphate (Merck, cat. no. 1.04873.1000) Salmon sperm DNA (Life Technologies, cat. no. 15632-011) Sodium chloride (Merck, cat. no. 1.06404.1000) Disodium hydrogen phosphate dihydrate (Merck, cat. no. 1.06580.1000) Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC; Thermo Scientific, cat. no. 22322) SYBR Green I nucleic acid gel stain, 10,000× concentrate in DMSO (Life Technologies, cat. no. S-7563) T4 DNA ligase, 1 U μl − 1 (Thermo Scientific, cat. no. EL0016) Tween-20 (Sigma-Aldrich, cat. no. P9416) Uracil-DNA glycosylase (UDG), 1 U μl − 1 (Thermo Scientific, cat. no. EN0362) UltraPure dithiothreitol (Life Technologies, cat. no. 15508-013) Zeba Spin desalting columns, 7 K MWCO, 0.5 ml (Thermo Scientific, cat. no. 89882) Zeba Spin desalting plates, 7 K MWCO (Thermo Scientific, cat. no. 89808) Trizma base EQUIPMENT Beaker, 5 liters PCR strip, eight wells (e.g., Applied Biosystems, cat. no. 4316567) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • PCR plate, 96 wells (e.g., Sarstedt, cat. no. 72.1978.202; Thermo Scientific, cat. no. 732-4828) 2720 thermal cycler (Applied Biosystems, cat. no. 4359659) 7900 HT fast real-time PCR system (Applied Biosystems, cat. no. 4329001) Cap strips (8 caps per strip; e.g., Applied Biosystems, cat. no. 4323032) DynaMag-2 magnet (Life Technologies, cat. no. 12321D), or similar magnetic stand for microcentrifuge tubes DynaMag-96 side, 96-well-plate magnet (Life Technologies, cat. no. 12331D) E-gel agarose gel electrophoresis system (Life Technologies, cat. no. G6500) E-gel precasted agarose gel (Life Technologies, cat. no. G402002) Hydra 96 system (Robbins Scientific) Icebox Magnetic stir bar MicroAmp optical 384-well reaction plate with bar code (Applied Biosystems, cat. no. 4309849) Multichannel pipette (Eppendorf International) M×3000P qPCR system (Agilent Technologies) Plate centrifuge (Eppendorf International) Rotator (Grant Bio PTR-30 360-degree vertical multifunction rotator, or similar) Self-adhesive PCR film (Sarstedt, cat. no. 95.1994) Slide-A-Lyzer MINI dialysis devices, 7 K MWCO (Thermo Scientific, cat. no. 69562) Vortex mixer REAGENT SETUP PBS buffer, 1×, pH 7.4 PBS buffer contains 137 mM NaCl, 2.7 mM KCl and 10 mM phosphate.
Transferring:Article Title: Solid-phase proximity ligation assays for individual or parallel protein analyses with readout via real-time PCR or sequencing.
Article Snippet: Streptavidin-conjugated oligonucleotides were ordered from Solulink PLA probes (see INTRODUCTION) Potassium acetate Potassium chloride (Merck, cat. no. 1.04936.1000) Potassium dihydrogen phosphate (Merck, cat. no. 1.04873.1000) Salmon sperm DNA (Life Technologies, cat. no. 15632-011) Sodium chloride (Merck, cat. no. 1.06404.1000) Disodium hydrogen phosphate dihydrate (Merck, cat. no. 1.06580.1000) Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC; Thermo Scientific, cat. no. 22322) SYBR Green I nucleic acid gel stain, 10,000× concentrate in DMSO (Life Technologies, cat. no. S-7563) T4 DNA ligase, 1 U μl − 1 (Thermo Scientific, cat. no. EL0016) Tween-20 (Sigma-Aldrich, cat. no. P9416) Uracil-DNA glycosylase (UDG), 1 U μl − 1 (Thermo Scientific, cat. no. EN0362) UltraPure dithiothreitol (Life Technologies, cat. no. 15508-013) Zeba Spin desalting columns, 7 K MWCO, 0.5 ml (Thermo Scientific, cat. no. 89882) Zeba Spin desalting plates, 7 K MWCO (Thermo Scientific, cat. no. 89808) Trizma base EQUIPMENT Beaker, 5 liters PCR strip, eight wells (e.g., Applied Biosystems, cat. no. 4316567) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • PCR plate, 96 wells (e.g., Sarstedt, cat. no. 72.1978.202; Thermo Scientific, cat. no. 732-4828) 2720 thermal cycler (Applied Biosystems, cat. no. 4359659) 7900 HT fast real-time PCR system (Applied Biosystems, cat. no. 4329001) Cap strips (8 caps per strip; e.g., Applied Biosystems, cat. no. 4323032) DynaMag-2 magnet (Life Technologies, cat. no. 12321D), or similar magnetic stand for microcentrifuge tubes DynaMag-96 side, 96-well-plate magnet (Life Technologies, cat. no. 12331D) E-gel agarose gel electrophoresis system (Life Technologies, cat. no. G6500) E-gel precasted agarose gel (Life Technologies, cat. no. G402002) Hydra 96 system (Robbins Scientific) Icebox Magnetic stir bar MicroAmp optical 384-well reaction plate with bar code (Applied Biosystems, cat. no. 4309849) Multichannel pipette (Eppendorf International) M×3000P qPCR system (Agilent Technologies) Plate centrifuge (Eppendorf International) Rotator (Grant Bio PTR-30 360-degree vertical multifunction rotator, or similar) Self-adhesive PCR film (Sarstedt, cat. no. 95.1994) Slide-A-Lyzer MINI dialysis devices, 7 K MWCO (Thermo Scientific, cat. no. 69562) Vortex mixer REAGENT SETUP PBS buffer, 1×, pH 7.4 PBS buffer contains 137 mM NaCl, 2.7 mM KCl and 10 mM phosphate.
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